Transgenic and Gene Targeting Core

Welcome to the Transgenic and Gene Targeting Core (TGT Core) at Georgia State University. The TGT Core is a research resource for investigators at Georgia State University as well as surrounding institutions to produce genetically engineered animal models.

Services

pronucleus
Microinjection of DNA constructs into the pronucleus of single-cell embryos
perivitelline
Microinjection of lentiviruses into perivitelline space of single-cell embryos
To request services, please fill out the forms and submit the completed and signed form to Core Director Dr. Chengliu Jin.
Blastocyst-microinjection-of-embryonic-stem-ES-cells
Blastocyst microinjection of embryonic stem (ES) cells
cytoplasmic
CRISPR/Cas9 Technology: Pronuclear or cytoplasmic microinjection of Cas9 mRNA, sgRNA and Donor DNA
To request services, please fill out the forms and submit the completed and signed form to Core Director Dr. Chengliu Jin.
This service is used to rederive animals via embryo transfer:

  • Rederivation from frozen embryos to recover the frozen stocks or to import animal strains
  • Rederivation from pathogen infected strains to eradicate unwanted infectious agents which may confound one’s animal research data

To request services, please fill out the forms and submit the completed and signed form to Core Director Core Director Dr. Chengliu Jin.

For information about the additional services or to discuss a desired project, please contact Core Director Dr. Chengliu Jin.

Fees & Forms

Service Description

GSU

Non-GSU

Plasmid DNA pronuclear microinjection

$1800

$2460

BAC DNA pronuclear microinjection

$2500

$3160

Lentiviruses microinjection

$1800

$2460

ES cell blastocyst microinjection

$1800

$2460

ES cell blastocyst microinjection (albino)

$1950

$2610

CRISPR/Cas9 microinjection (knock-out)

$1800

$2460

CRISPR/Cas9 microinjection (knock-in)

$2500

$3160

Rederivation (frozen embryos)

$600

$765

Rederivation (pathogen infection strains)

$1000

$1165

ES cell recovery, expansion and preservation

$60/hr

$80/hr

Contact Information

Chengliu Jin, Ph.D.
Director, Transgenic and Gene Targeting Core
Georgia State University
701 Petit Science Center
100 Piedmont Ave SE
Atlanta, GA 30303
E-mail: cjin5@gsu.edu or transgenic@gsu.edu
Phone: (404) 413-3603
Fax: (404) 413-5471

Qian (Zoe) Liu, M.S.
Lab Technician
Phone: (678)517-2290
Email: qliu4@gsu.edu

Transgenic and Gene Targeting Core
Georgia State University
940 Petit Science Center
100 Piedmont Ave SE
Atlanta, GA 30303

Mailing Address
Division of Animal Resources
P.O. Box 4010
Atlanta, GA 30302-4010

Shipping Address
Petit Science Center
161 Jesse Hill Jr. Dr. Se
Atlanta, GA 30303-3222